Analyses

Old USA UgLab tests

When we analysed samples from Underground Laboratories, a member of our forum asked me if I wanted to do a  "special" on American samples. The market in the US is special from most parts of the world, the generic domestic American market that is. Because the Law Enforcement is so unbelievable strict on PED's (performence enhancing drugs) in street slang steroids, roids or anabolics the brands and sellers fluctuate more often than in other parts of the world.

This should be especially interesting for some US bodybuilders that know one or more of the analysed labs.

Since lots of analyses disappaered from the net since Body of Science forum is closed, some previous Mods of this notorious forum asked me to make them available again I have an enormous amount of analyses that I still have to open-scan and tag, but slowly I'll post then here. Four to start with!!

Quality and doses in basic analysis methods

Basics of QC of oil-based parenterals.

Well, everybody knows that steroid based injections are faked. We will pass by the meter why it happens and will discuss how it happens and what could we do to avoid fakes. First of I would like to show you how the oil-based parentals are normally made. After that I’ll try to discover basics of quality testing and some advanced things in brief.

And an Other (bad) Chinese IGF-1 LR3 sample

In the previous issue we published an other analysis of a IGF-1 LR3 sample we bought in the Netherlands and that originated from China.

It turned out it was a very dangerous item that contained ratinsulin instead of IGF-1 LR3!!

When we started to analyse a new sample we bought in China, we found  it was IGF-1 this time.

Analysis of CJC-1295 with attached Drug Affinity Complex.

GHRH is a growth hormone releasing factor peptide that is comprised of either 44 or 40 amino acids and is abbreviated as GRF(1-29). The biologically active portion resides in the first 29 amino acids so the synthetic forms of GHRH are made up of just these 29 amino acids.

CJC-1295 has an added amino acid lysine which links to a drug affinity complex (DAC). CJC 1295 without DAC is modified GHRH or GRF(1-29).

Modified GRF(1-29) is a short-estered GHRH, while CJC-1295 is a long-estered GHRH. Effectively, the long-estered GHRH can be run once a week if you can find the real deal.
Since maufacturing CJC 1295 is a complex and costly process, most all CJC 1295 being currently sold is really GRF(1-29). This analysis confirmed this assumption!!

Its very profitable to buy GRF as middleman and sell them as CJC 1295-DAC

Real and fake Norditropin Simplexx

There where many discussions about on many fora about counterfeited Nordiropin Simplexx and members conpared pictures and details from their products. One of our members was convinced he had bought some counterfeits. I asked our peptide lab to run some analyses. It turned out it was  a real item.

Analysis of an MT2 (Melatonan 2) peptide.

One of our members used this petide to get a nice tan. It’s a MT2 with a nice box with hologram. The top (with brand name), label and box are made in the same colour with great precision and it looks like a pharmaceutical item. The brandname and info are not traceble on the internet, thus it concerns a underground made product, most probably originating from China or India. Our member was very curious to its quality. That’s why I decided to run an analysis.

The brandname is Scenic batchnumber: 000001380 expiring: 01 december 2010

As you can easily see, the main compound has a MW  (Molecular Weight) of 1024.5 Da (Dalton) which is in accordance with the cyclic peptide.

 

However, there are a high number of minor contaminations in the sample which you see better in the zoom pictures.

IGF-1 LR3 analysis.

 

One of my friends, a huge guy who competes in the strongest man competition, had bought some IGF-1 vials. The brand name was Nutral with lot number 050303. According to the label it contained 1000 mcg IGF-1 LR3 in acetic acid. He lifted his shirt and showed me his body. He had blue coloured bruises alike spots on his body the size of a hand, with fluid retention underneath. Later he confessed that they had lasted for weeks. I became very curious and tried to find a laboratory that had the capability to analyse complex proteins. I found one in Germany.

GenShi Masteron analyse

A few of our members asked to post some analytical reports. There are a few ways to analyse a product in this case its fully analysed. They looked for water (Karl Fisher) mostly present if the API (active pharmaceutical ingredient aka raw powders) is not stored properly. The Ph (accidity) mostly due to unpure powders. The identity (if the compound is the compound labeled) in this case does the sample contain drostenolone propionate. The dose (the amount of active ingredients per milliliter). The heavy metals (those can build up in the body) We thought that most API's from India and China would possess a high content of metals -acids etc. That was the  main reason to analyse Underground laboratories.

IGF-1 LR3 analysis

 

“The Blue Tops”: IGF-1 LR3
by Ronny Tober

In the previous issue of Body of Science we published an other analysis of a IGF-1 LR3 sample we bought in the Netherlands and that originated from China. It turned out it was a very dangerous item that contained rat insulin instead of IGF-1 LR3! This time we are back doing protein analysis. In this case, we are again looking at IGF-1 LongR3. We obtained this vial of IGF-1LR3 from China, the principle source country of all IGF-1LR3. We did our best to pick a product that was popular, and thus was representative of the quality of many IGF-1LR3 samples. The results were, again, concerning.

German Black Market analyses

 Counterfeited steroids a problem since years.

 Counterfeited anabolic-androgenic steroids are a problem as long as I can remember.  Already in the nineties almost half of all the steroids on the black market pinched.  That appeared clearly from an analysis of preparations from the nineties, that was published is in 2000 in the German periodical Sportverletzung Sportschaden. 

 The investigation had relation on forty compoundss, that all were originating from the black market in Germany.  It does not concern samples that where bought and examined in the years 2000 or in 1999.  Already in 1997 the same researchers published these results, in another magazine.(2) Because between the publishing of an article in a scientific periodical and the date of publication sometimes a year is well possible, you can assume that these anabolics have been collected early  1996.  Experts, that on our request looked at the testresults, think that this study gives a clear image of the situation halfway the nineties.  Take by example those Parabolans'', says a consulted anabolic expert.  ,, You stumbled about  those in 1997 .  They were pinched, but you could buy them everywhere .  If you  ask me: what were 'the' means of the nineties, then I would say:  Paynes-deca, Thaibol and pinched-Parabolan